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tcf4 knockdown plasmid  (Addgene inc)


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    Addgene inc tcf4 knockdown plasmid
    <t>TCF4</t> promotes the increased generation of deep-layer neurons. (A) Schematic diagram of TCF4 overexpression clone construction. (B) Western blot validation of TCF4 overexpression clone efficiency. β-actin is as the reference protein. (C-H) pCIG+pCAG (control) and pCIG- Tcf4 +pCAG (TCF4) were electroporated into embryonic brains from mice at E13.5. Brain sections at P3 were immunostained with cortical markers (CUX1 and SOX5). Scale bar: 500 µm (C, D) and 100 µm (E-H, E'-H'). (I) The sections labelled A' to B' were divided into 10 bins to count the distribution of EGFP-positive cells in the cortex. These sections were derived from the electroporated mouse brains, which were from different littermates (control: n=15 sections from 13 brains, TCF4: n=4). (J) Statistical analysis of the percentage of CUX1+/GFP+ cells in (E', F') and SOX5+/GFP+ cells in (G', H') (control: n = 15 sections from 13 brains, TCF4: n=4). Statistical significance was determined using an unpaired two-tailed Student's t-test. *P<0.05, ** P<0.01 and ***P<0.001
    Tcf4 Knockdown Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tcf4 knockdown plasmid/product/Addgene inc
    Average 88 stars, based on 6 article reviews
    tcf4 knockdown plasmid - by Bioz Stars, 2026-05
    88/100 stars

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    1) Product Images from "MicroRNA-495 Modulates Neuronal Layer Fate Determination by Targeting Tcf4"

    Article Title: MicroRNA-495 Modulates Neuronal Layer Fate Determination by Targeting Tcf4

    Journal: International Journal of Biological Sciences

    doi: 10.7150/ijbs.94739

    TCF4 promotes the increased generation of deep-layer neurons. (A) Schematic diagram of TCF4 overexpression clone construction. (B) Western blot validation of TCF4 overexpression clone efficiency. β-actin is as the reference protein. (C-H) pCIG+pCAG (control) and pCIG- Tcf4 +pCAG (TCF4) were electroporated into embryonic brains from mice at E13.5. Brain sections at P3 were immunostained with cortical markers (CUX1 and SOX5). Scale bar: 500 µm (C, D) and 100 µm (E-H, E'-H'). (I) The sections labelled A' to B' were divided into 10 bins to count the distribution of EGFP-positive cells in the cortex. These sections were derived from the electroporated mouse brains, which were from different littermates (control: n=15 sections from 13 brains, TCF4: n=4). (J) Statistical analysis of the percentage of CUX1+/GFP+ cells in (E', F') and SOX5+/GFP+ cells in (G', H') (control: n = 15 sections from 13 brains, TCF4: n=4). Statistical significance was determined using an unpaired two-tailed Student's t-test. *P<0.05, ** P<0.01 and ***P<0.001
    Figure Legend Snippet: TCF4 promotes the increased generation of deep-layer neurons. (A) Schematic diagram of TCF4 overexpression clone construction. (B) Western blot validation of TCF4 overexpression clone efficiency. β-actin is as the reference protein. (C-H) pCIG+pCAG (control) and pCIG- Tcf4 +pCAG (TCF4) were electroporated into embryonic brains from mice at E13.5. Brain sections at P3 were immunostained with cortical markers (CUX1 and SOX5). Scale bar: 500 µm (C, D) and 100 µm (E-H, E'-H'). (I) The sections labelled A' to B' were divided into 10 bins to count the distribution of EGFP-positive cells in the cortex. These sections were derived from the electroporated mouse brains, which were from different littermates (control: n=15 sections from 13 brains, TCF4: n=4). (J) Statistical analysis of the percentage of CUX1+/GFP+ cells in (E', F') and SOX5+/GFP+ cells in (G', H') (control: n = 15 sections from 13 brains, TCF4: n=4). Statistical significance was determined using an unpaired two-tailed Student's t-test. *P<0.05, ** P<0.01 and ***P<0.001

    Techniques Used: Over Expression, Western Blot, Biomarker Discovery, Control, Derivative Assay, Two Tailed Test

    TCF4 knockdown restrains the generation of deep-layer neurons. (A) Schematic diagram of TCF4 knockdown clone construction. The designations sh1, sh2, and sh3 refer to pLL3.7-sh1- Tcf4 , pLL3.7-sh2- Tcf4 , and pLL3.7-sh3- Tcf4 , respectively. (B) WB validation of TCF4 knockdown clone efficiency. β-actin is as the reference protein. (C-K) pLL3.7-Scr+pCAG, pLL3.7-sh1- Tcf4 + pCAG and pLL3.7-sh2- Tcf4 + pCAG were electroporated into the embryonic brains of mice at E13.5. Brain sections at P3 were immunostained with cortical markers (CUX1 and SOX5). Scale bar: 500 µm (C, D, E) and 100 µm (F-K, F'-K') . (L) The sections labelled F to H were divided into 10 bins to count the distribution of EGFP-positive cells in the cortex. These sections were derived from the electroporated mouse brains, which were from different littermates (Scr: n=3, sh1-Tcf4: n=3, sh2-Tcf4: n=3). (M) The percentage of CUX1+/GFP+ cells and SOX5+/GFP+ cells in (F-K) (Scr: n=3, sh1-Tcf4: n=3, sh2-Tcf4: n=3). Statistical significance was determined using an unpaired two-tailed Student's t-test. Results are expressed as the mean ±SD. P values are shown as *P<0.05, ** P<0.01.
    Figure Legend Snippet: TCF4 knockdown restrains the generation of deep-layer neurons. (A) Schematic diagram of TCF4 knockdown clone construction. The designations sh1, sh2, and sh3 refer to pLL3.7-sh1- Tcf4 , pLL3.7-sh2- Tcf4 , and pLL3.7-sh3- Tcf4 , respectively. (B) WB validation of TCF4 knockdown clone efficiency. β-actin is as the reference protein. (C-K) pLL3.7-Scr+pCAG, pLL3.7-sh1- Tcf4 + pCAG and pLL3.7-sh2- Tcf4 + pCAG were electroporated into the embryonic brains of mice at E13.5. Brain sections at P3 were immunostained with cortical markers (CUX1 and SOX5). Scale bar: 500 µm (C, D, E) and 100 µm (F-K, F'-K') . (L) The sections labelled F to H were divided into 10 bins to count the distribution of EGFP-positive cells in the cortex. These sections were derived from the electroporated mouse brains, which were from different littermates (Scr: n=3, sh1-Tcf4: n=3, sh2-Tcf4: n=3). (M) The percentage of CUX1+/GFP+ cells and SOX5+/GFP+ cells in (F-K) (Scr: n=3, sh1-Tcf4: n=3, sh2-Tcf4: n=3). Statistical significance was determined using an unpaired two-tailed Student's t-test. Results are expressed as the mean ±SD. P values are shown as *P<0.05, ** P<0.01.

    Techniques Used: Knockdown, Biomarker Discovery, Derivative Assay, Two Tailed Test



    Similar Products

    tcf4 knockdown plasmid  (Addgene inc)
    88
    Addgene inc tcf4 knockdown plasmid
    <t>TCF4</t> promotes the increased generation of deep-layer neurons. (A) Schematic diagram of TCF4 overexpression clone construction. (B) Western blot validation of TCF4 overexpression clone efficiency. β-actin is as the reference protein. (C-H) pCIG+pCAG (control) and pCIG- Tcf4 +pCAG (TCF4) were electroporated into embryonic brains from mice at E13.5. Brain sections at P3 were immunostained with cortical markers (CUX1 and SOX5). Scale bar: 500 µm (C, D) and 100 µm (E-H, E'-H'). (I) The sections labelled A' to B' were divided into 10 bins to count the distribution of EGFP-positive cells in the cortex. These sections were derived from the electroporated mouse brains, which were from different littermates (control: n=15 sections from 13 brains, TCF4: n=4). (J) Statistical analysis of the percentage of CUX1+/GFP+ cells in (E', F') and SOX5+/GFP+ cells in (G', H') (control: n = 15 sections from 13 brains, TCF4: n=4). Statistical significance was determined using an unpaired two-tailed Student's t-test. *P<0.05, ** P<0.01 and ***P<0.001
    Tcf4 Knockdown Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tcf4 knockdown plasmid/product/Addgene inc
    Average 88 stars, based on 1 article reviews
    tcf4 knockdown plasmid - by Bioz Stars, 2026-05
    88/100 stars
    		  Buy from Supplier

    Image Search Results


    TCF4 promotes the increased generation of deep-layer neurons. (A) Schematic diagram of TCF4 overexpression clone construction. (B) Western blot validation of TCF4 overexpression clone efficiency. β-actin is as the reference protein. (C-H) pCIG+pCAG (control) and pCIG- Tcf4 +pCAG (TCF4) were electroporated into embryonic brains from mice at E13.5. Brain sections at P3 were immunostained with cortical markers (CUX1 and SOX5). Scale bar: 500 µm (C, D) and 100 µm (E-H, E'-H'). (I) The sections labelled A' to B' were divided into 10 bins to count the distribution of EGFP-positive cells in the cortex. These sections were derived from the electroporated mouse brains, which were from different littermates (control: n=15 sections from 13 brains, TCF4: n=4). (J) Statistical analysis of the percentage of CUX1+/GFP+ cells in (E', F') and SOX5+/GFP+ cells in (G', H') (control: n = 15 sections from 13 brains, TCF4: n=4). Statistical significance was determined using an unpaired two-tailed Student's t-test. *P<0.05, ** P<0.01 and ***P<0.001

    Journal: International Journal of Biological Sciences

    Article Title: MicroRNA-495 Modulates Neuronal Layer Fate Determination by Targeting Tcf4

    doi: 10.7150/ijbs.94739

    Figure Lengend Snippet: TCF4 promotes the increased generation of deep-layer neurons. (A) Schematic diagram of TCF4 overexpression clone construction. (B) Western blot validation of TCF4 overexpression clone efficiency. β-actin is as the reference protein. (C-H) pCIG+pCAG (control) and pCIG- Tcf4 +pCAG (TCF4) were electroporated into embryonic brains from mice at E13.5. Brain sections at P3 were immunostained with cortical markers (CUX1 and SOX5). Scale bar: 500 µm (C, D) and 100 µm (E-H, E'-H'). (I) The sections labelled A' to B' were divided into 10 bins to count the distribution of EGFP-positive cells in the cortex. These sections were derived from the electroporated mouse brains, which were from different littermates (control: n=15 sections from 13 brains, TCF4: n=4). (J) Statistical analysis of the percentage of CUX1+/GFP+ cells in (E', F') and SOX5+/GFP+ cells in (G', H') (control: n = 15 sections from 13 brains, TCF4: n=4). Statistical significance was determined using an unpaired two-tailed Student's t-test. *P<0.05, ** P<0.01 and ***P<0.001

    Article Snippet: The TCF4 knockdown plasmid was constructed using the pLL3.7 vector (Addgene).

    Techniques: Over Expression, Western Blot, Biomarker Discovery, Control, Derivative Assay, Two Tailed Test

    TCF4 knockdown restrains the generation of deep-layer neurons. (A) Schematic diagram of TCF4 knockdown clone construction. The designations sh1, sh2, and sh3 refer to pLL3.7-sh1- Tcf4 , pLL3.7-sh2- Tcf4 , and pLL3.7-sh3- Tcf4 , respectively. (B) WB validation of TCF4 knockdown clone efficiency. β-actin is as the reference protein. (C-K) pLL3.7-Scr+pCAG, pLL3.7-sh1- Tcf4 + pCAG and pLL3.7-sh2- Tcf4 + pCAG were electroporated into the embryonic brains of mice at E13.5. Brain sections at P3 were immunostained with cortical markers (CUX1 and SOX5). Scale bar: 500 µm (C, D, E) and 100 µm (F-K, F'-K') . (L) The sections labelled F to H were divided into 10 bins to count the distribution of EGFP-positive cells in the cortex. These sections were derived from the electroporated mouse brains, which were from different littermates (Scr: n=3, sh1-Tcf4: n=3, sh2-Tcf4: n=3). (M) The percentage of CUX1+/GFP+ cells and SOX5+/GFP+ cells in (F-K) (Scr: n=3, sh1-Tcf4: n=3, sh2-Tcf4: n=3). Statistical significance was determined using an unpaired two-tailed Student's t-test. Results are expressed as the mean ±SD. P values are shown as *P<0.05, ** P<0.01.

    Journal: International Journal of Biological Sciences

    Article Title: MicroRNA-495 Modulates Neuronal Layer Fate Determination by Targeting Tcf4

    doi: 10.7150/ijbs.94739

    Figure Lengend Snippet: TCF4 knockdown restrains the generation of deep-layer neurons. (A) Schematic diagram of TCF4 knockdown clone construction. The designations sh1, sh2, and sh3 refer to pLL3.7-sh1- Tcf4 , pLL3.7-sh2- Tcf4 , and pLL3.7-sh3- Tcf4 , respectively. (B) WB validation of TCF4 knockdown clone efficiency. β-actin is as the reference protein. (C-K) pLL3.7-Scr+pCAG, pLL3.7-sh1- Tcf4 + pCAG and pLL3.7-sh2- Tcf4 + pCAG were electroporated into the embryonic brains of mice at E13.5. Brain sections at P3 were immunostained with cortical markers (CUX1 and SOX5). Scale bar: 500 µm (C, D, E) and 100 µm (F-K, F'-K') . (L) The sections labelled F to H were divided into 10 bins to count the distribution of EGFP-positive cells in the cortex. These sections were derived from the electroporated mouse brains, which were from different littermates (Scr: n=3, sh1-Tcf4: n=3, sh2-Tcf4: n=3). (M) The percentage of CUX1+/GFP+ cells and SOX5+/GFP+ cells in (F-K) (Scr: n=3, sh1-Tcf4: n=3, sh2-Tcf4: n=3). Statistical significance was determined using an unpaired two-tailed Student's t-test. Results are expressed as the mean ±SD. P values are shown as *P<0.05, ** P<0.01.

    Article Snippet: The TCF4 knockdown plasmid was constructed using the pLL3.7 vector (Addgene).

    Techniques: Knockdown, Biomarker Discovery, Derivative Assay, Two Tailed Test